mesc cell culture medium Search Results


cell lines wt r1 mesc atcc cat  (ATCC)
94
ATCC cell lines wt r1 mesc atcc cat
Cell Lines Wt R1 Mesc Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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rpmi 1640 medium  (Gold Biotechnology Inc)
96
Gold Biotechnology Inc rpmi 1640 medium
Rpmi 1640 Medium, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
rpmi 1640 medium - by Bioz Stars, 2026-04
96/100 stars
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growth medium  (Cell Applications Inc)
96
Cell Applications Inc growth medium
Growth Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
growth medium - by Bioz Stars, 2026-04
96/100 stars
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rpmi-1640 medium  (ICN Pharmaceuticals)
90
ICN Pharmaceuticals rpmi-1640 medium
Rpmi 1640 Medium, supplied by ICN Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90/100 stars
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mescs culture medium  (Cyagen Biosciences)
90
Cyagen Biosciences mescs culture medium
Mescs Culture Medium, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mescs culture medium - by Bioz Stars, 2026-04
90/100 stars
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dulbecco’s modified eagle’s medium  (Thermo Fisher)
90
Thermo Fisher dulbecco’s modified eagle’s medium
Dulbecco’s Modified Eagle’s Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
dulbecco’s modified eagle’s medium - by Bioz Stars, 2026-04
90/100 stars
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rpmi 1640 medium  (Flow Laboratories)
90
Flow Laboratories rpmi 1640 medium
Rpmi 1640 Medium, supplied by Flow Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rpmi 1640 medium - by Bioz Stars, 2026-04
90/100 stars
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mes medium (dmem  (Millipore)
90
Millipore mes medium (dmem
Mes Medium (Dmem, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mes medium (dmem - by Bioz Stars, 2026-04
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mesothelin meso scale assay  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC mesothelin meso scale assay
A : KLM-1 and resistant KLM-1 (KLM-1-R) cells were incubated for 72 hrs with the <t>anti-mesothelin</t> SS1-LR-GGS, RG7787 or anti-CD25 LMB-2 as a control. Growth inhibition was evaluated with an ATP cell viability assay. With IC 50 s below 10 ng/ml, KLM-1 is sensitive to the anti-mesothelin RITs, which is not the case for KLM-1-R (IC 50 s > 1 μg/ml). 1 μg/ml LMB-2 decreased cell viability, indicating that this RIT concentration induces non-specific uptake. B : KLM-1 and KLM-1-R cells were incubated for 72 hrs with 100 or 1000 ng/ml RG7787. Apoptosis was evaluated with the Annexin V-PE Apoptosis Detection Kit I. RG7787 induces a significant increase in apoptotic KLM-1 cells, whereas KLM-1-R cells shows no meaningful increase in apoptosis. C : KLM-1 and KLM-1-R cells were incubated for 72 hrs with HB21(Fv)-PE40. Growth inhibition was evaluated with an ATP cell viability assay. Both cell lines are highly sensitive to this RIT.
Mesothelin Meso Scale Assay, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mesothelin meso scale assay/product/Meso Scale Diagnostics LLC
Average 90 stars, based on 1 article reviews
mesothelin meso scale assay - by Bioz Stars, 2026-04
90/100 stars
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rpmi c rpmi rpmi 1640 medium supplemented with 2 mm l glutamine 25 mm hepes  (Thermo Fisher)
99
Thermo Fisher rpmi c rpmi rpmi 1640 medium supplemented with 2 mm l glutamine 25 mm hepes
A : KLM-1 and resistant KLM-1 (KLM-1-R) cells were incubated for 72 hrs with the <t>anti-mesothelin</t> SS1-LR-GGS, RG7787 or anti-CD25 LMB-2 as a control. Growth inhibition was evaluated with an ATP cell viability assay. With IC 50 s below 10 ng/ml, KLM-1 is sensitive to the anti-mesothelin RITs, which is not the case for KLM-1-R (IC 50 s > 1 μg/ml). 1 μg/ml LMB-2 decreased cell viability, indicating that this RIT concentration induces non-specific uptake. B : KLM-1 and KLM-1-R cells were incubated for 72 hrs with 100 or 1000 ng/ml RG7787. Apoptosis was evaluated with the Annexin V-PE Apoptosis Detection Kit I. RG7787 induces a significant increase in apoptotic KLM-1 cells, whereas KLM-1-R cells shows no meaningful increase in apoptosis. C : KLM-1 and KLM-1-R cells were incubated for 72 hrs with HB21(Fv)-PE40. Growth inhibition was evaluated with an ATP cell viability assay. Both cell lines are highly sensitive to this RIT.
Rpmi C Rpmi Rpmi 1640 Medium Supplemented With 2 Mm L Glutamine 25 Mm Hepes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rpmi c rpmi rpmi 1640 medium supplemented with 2 mm l glutamine 25 mm hepes/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
rpmi c rpmi rpmi 1640 medium supplemented with 2 mm l glutamine 25 mm hepes - by Bioz Stars, 2026-04
99/100 stars
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meso scale discovery sector imager 2400  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC meso scale discovery sector imager 2400
A : KLM-1 and resistant KLM-1 (KLM-1-R) cells were incubated for 72 hrs with the <t>anti-mesothelin</t> SS1-LR-GGS, RG7787 or anti-CD25 LMB-2 as a control. Growth inhibition was evaluated with an ATP cell viability assay. With IC 50 s below 10 ng/ml, KLM-1 is sensitive to the anti-mesothelin RITs, which is not the case for KLM-1-R (IC 50 s > 1 μg/ml). 1 μg/ml LMB-2 decreased cell viability, indicating that this RIT concentration induces non-specific uptake. B : KLM-1 and KLM-1-R cells were incubated for 72 hrs with 100 or 1000 ng/ml RG7787. Apoptosis was evaluated with the Annexin V-PE Apoptosis Detection Kit I. RG7787 induces a significant increase in apoptotic KLM-1 cells, whereas KLM-1-R cells shows no meaningful increase in apoptosis. C : KLM-1 and KLM-1-R cells were incubated for 72 hrs with HB21(Fv)-PE40. Growth inhibition was evaluated with an ATP cell viability assay. Both cell lines are highly sensitive to this RIT.
Meso Scale Discovery Sector Imager 2400, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/meso scale discovery sector imager 2400/product/Meso Scale Diagnostics LLC
Average 90 stars, based on 1 article reviews
meso scale discovery sector imager 2400 - by Bioz Stars, 2026-04
90/100 stars
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mouse mesangial cells mes 13  (ATCC)
96
ATCC mouse mesangial cells mes 13
A : KLM-1 and resistant KLM-1 (KLM-1-R) cells were incubated for 72 hrs with the <t>anti-mesothelin</t> SS1-LR-GGS, RG7787 or anti-CD25 LMB-2 as a control. Growth inhibition was evaluated with an ATP cell viability assay. With IC 50 s below 10 ng/ml, KLM-1 is sensitive to the anti-mesothelin RITs, which is not the case for KLM-1-R (IC 50 s > 1 μg/ml). 1 μg/ml LMB-2 decreased cell viability, indicating that this RIT concentration induces non-specific uptake. B : KLM-1 and KLM-1-R cells were incubated for 72 hrs with 100 or 1000 ng/ml RG7787. Apoptosis was evaluated with the Annexin V-PE Apoptosis Detection Kit I. RG7787 induces a significant increase in apoptotic KLM-1 cells, whereas KLM-1-R cells shows no meaningful increase in apoptosis. C : KLM-1 and KLM-1-R cells were incubated for 72 hrs with HB21(Fv)-PE40. Growth inhibition was evaluated with an ATP cell viability assay. Both cell lines are highly sensitive to this RIT.
Mouse Mesangial Cells Mes 13, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse mesangial cells mes 13/product/ATCC
Average 96 stars, based on 1 article reviews
mouse mesangial cells mes 13 - by Bioz Stars, 2026-04
96/100 stars
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Image Search Results


A : KLM-1 and resistant KLM-1 (KLM-1-R) cells were incubated for 72 hrs with the anti-mesothelin SS1-LR-GGS, RG7787 or anti-CD25 LMB-2 as a control. Growth inhibition was evaluated with an ATP cell viability assay. With IC 50 s below 10 ng/ml, KLM-1 is sensitive to the anti-mesothelin RITs, which is not the case for KLM-1-R (IC 50 s > 1 μg/ml). 1 μg/ml LMB-2 decreased cell viability, indicating that this RIT concentration induces non-specific uptake. B : KLM-1 and KLM-1-R cells were incubated for 72 hrs with 100 or 1000 ng/ml RG7787. Apoptosis was evaluated with the Annexin V-PE Apoptosis Detection Kit I. RG7787 induces a significant increase in apoptotic KLM-1 cells, whereas KLM-1-R cells shows no meaningful increase in apoptosis. C : KLM-1 and KLM-1-R cells were incubated for 72 hrs with HB21(Fv)-PE40. Growth inhibition was evaluated with an ATP cell viability assay. Both cell lines are highly sensitive to this RIT.

Journal: PLoS ONE

Article Title: Methylation-Associated Partial Down-Regulation of Mesothelin Causes Resistance to Anti-Mesothelin Immunotoxins in a Pancreatic Cancer Cell Line

doi: 10.1371/journal.pone.0122462

Figure Lengend Snippet: A : KLM-1 and resistant KLM-1 (KLM-1-R) cells were incubated for 72 hrs with the anti-mesothelin SS1-LR-GGS, RG7787 or anti-CD25 LMB-2 as a control. Growth inhibition was evaluated with an ATP cell viability assay. With IC 50 s below 10 ng/ml, KLM-1 is sensitive to the anti-mesothelin RITs, which is not the case for KLM-1-R (IC 50 s > 1 μg/ml). 1 μg/ml LMB-2 decreased cell viability, indicating that this RIT concentration induces non-specific uptake. B : KLM-1 and KLM-1-R cells were incubated for 72 hrs with 100 or 1000 ng/ml RG7787. Apoptosis was evaluated with the Annexin V-PE Apoptosis Detection Kit I. RG7787 induces a significant increase in apoptotic KLM-1 cells, whereas KLM-1-R cells shows no meaningful increase in apoptosis. C : KLM-1 and KLM-1-R cells were incubated for 72 hrs with HB21(Fv)-PE40. Growth inhibition was evaluated with an ATP cell viability assay. Both cell lines are highly sensitive to this RIT.

Article Snippet: Soluble mesothelin levels in cell line medium were measured in duplicate with a mesothelin Meso Scale Assay (Morphotek, Inc.), using the electrochemiluminescence technology of Meso Scale Discovery.

Techniques: Incubation, Inhibition, Viability Assay, Concentration Assay

A : Surface mesothelin levels are 5-fold lower in resistant KLM-1 (KLM-1-R) compared to KLM-1. Mesothelin expression of KLM-1, KLM-1-R and mesothelin-negative A431 cells (negative control) were evaluated using flow cytometry. Filled histograms are secondary antibody controls. B : Whole mesothelin protein level is decreased in KLM-1-R. Precursor (72 kDa) and cleaved mature mesothelin (37 kDa) were present in KLM-1. In KLM-1-R, the cleaved portion was detected at low levels. Protein levels were probed in untreated KLM-1 and KLM-1-R cell lysate by Western blot. β-actin acts as loading control. C : At each time point, cellular uptake of RG7787-Alexa647 in KLM-1 is significantly higher than in KLM-1-R, and significantly lower than in KLM-1-R- Msln (transfected with mesothelin). Uptake was evaluated at 30, 75 and 150 min. Average geomean fluorescence intensities converted into amount of RG7787 molecules.

Journal: PLoS ONE

Article Title: Methylation-Associated Partial Down-Regulation of Mesothelin Causes Resistance to Anti-Mesothelin Immunotoxins in a Pancreatic Cancer Cell Line

doi: 10.1371/journal.pone.0122462

Figure Lengend Snippet: A : Surface mesothelin levels are 5-fold lower in resistant KLM-1 (KLM-1-R) compared to KLM-1. Mesothelin expression of KLM-1, KLM-1-R and mesothelin-negative A431 cells (negative control) were evaluated using flow cytometry. Filled histograms are secondary antibody controls. B : Whole mesothelin protein level is decreased in KLM-1-R. Precursor (72 kDa) and cleaved mature mesothelin (37 kDa) were present in KLM-1. In KLM-1-R, the cleaved portion was detected at low levels. Protein levels were probed in untreated KLM-1 and KLM-1-R cell lysate by Western blot. β-actin acts as loading control. C : At each time point, cellular uptake of RG7787-Alexa647 in KLM-1 is significantly higher than in KLM-1-R, and significantly lower than in KLM-1-R- Msln (transfected with mesothelin). Uptake was evaluated at 30, 75 and 150 min. Average geomean fluorescence intensities converted into amount of RG7787 molecules.

Article Snippet: Soluble mesothelin levels in cell line medium were measured in duplicate with a mesothelin Meso Scale Assay (Morphotek, Inc.), using the electrochemiluminescence technology of Meso Scale Discovery.

Techniques: Expressing, Negative Control, Flow Cytometry, Western Blot, Transfection, Fluorescence

A : AZA leads to a 2.8-fold increase of mesothelin surface expression in resistant KLM-1 (KLM-1-R). Flow cytometry histogram of KLM-1, KLM-1-R, and KLM-1-R-AZA. Filled histograms represent secondary antibody controls. B : Three weeks of incubation with AZA, a DNA methyltransferase inhibitor, increases sensitivity to RG7787. KLM-1, KLM-1-R, and the AZA-treated cells (KLM-1-AZA and KLM-1-R-AZA) were treated for 72 hrs with RG7787. Growth inhibition was evaluated with an ATP cell viability assay. Dotted lines represent AZA-treated cells. C : CpGs in a region upstream of the mesothelin transcription start site are more methylated in KLM-1-R than in KLM-1. Three weeks of incubation with AZA decreases methylation in KLM-1-R cells. The analyzed region is located at chr16:808890-808742 and spans 147 bp and seven CpGs. D : Mesothelin transfection in KLM-1-R results in significant overexpression of mesothelin compared to KLM-1 (5-fold) and KLM-1-R (23-fold). Flow cytometry surface mesothelin levels in KLM-1, KLM-1-R and mesothelin-transfected resistant cells (KLM-1-R- Msln ). E : Mesothelin overexpression in KLM-1-R restores sensitivity to RG7787. KLM-1 and KLM-1-R- Msln cells were incubated for 72 hrs with RG7787. Growth inhibition was evaluated with an ATP cell viability assay.

Journal: PLoS ONE

Article Title: Methylation-Associated Partial Down-Regulation of Mesothelin Causes Resistance to Anti-Mesothelin Immunotoxins in a Pancreatic Cancer Cell Line

doi: 10.1371/journal.pone.0122462

Figure Lengend Snippet: A : AZA leads to a 2.8-fold increase of mesothelin surface expression in resistant KLM-1 (KLM-1-R). Flow cytometry histogram of KLM-1, KLM-1-R, and KLM-1-R-AZA. Filled histograms represent secondary antibody controls. B : Three weeks of incubation with AZA, a DNA methyltransferase inhibitor, increases sensitivity to RG7787. KLM-1, KLM-1-R, and the AZA-treated cells (KLM-1-AZA and KLM-1-R-AZA) were treated for 72 hrs with RG7787. Growth inhibition was evaluated with an ATP cell viability assay. Dotted lines represent AZA-treated cells. C : CpGs in a region upstream of the mesothelin transcription start site are more methylated in KLM-1-R than in KLM-1. Three weeks of incubation with AZA decreases methylation in KLM-1-R cells. The analyzed region is located at chr16:808890-808742 and spans 147 bp and seven CpGs. D : Mesothelin transfection in KLM-1-R results in significant overexpression of mesothelin compared to KLM-1 (5-fold) and KLM-1-R (23-fold). Flow cytometry surface mesothelin levels in KLM-1, KLM-1-R and mesothelin-transfected resistant cells (KLM-1-R- Msln ). E : Mesothelin overexpression in KLM-1-R restores sensitivity to RG7787. KLM-1 and KLM-1-R- Msln cells were incubated for 72 hrs with RG7787. Growth inhibition was evaluated with an ATP cell viability assay.

Article Snippet: Soluble mesothelin levels in cell line medium were measured in duplicate with a mesothelin Meso Scale Assay (Morphotek, Inc.), using the electrochemiluminescence technology of Meso Scale Discovery.

Techniques: Expressing, Flow Cytometry, Incubation, Inhibition, Viability Assay, Methylation, Transfection, Over Expression